Cancer its efficacy. Therefore, in this study, we

Cancer is one of the greatest
threats to human health, which has a high mortality and hard to treat. Standard
cancer treatments like surgery or chemo-, radiation, and hormone therapies
failing patients as it’s always accompanied by undesirable side effects. Therefore,
new therapeutic options for cancer treatment are highly needed. Peptide
therapeutics is a promising field for emerging anticancer agents because peptides are readily synthesized in a cost
efficient manner and are amenable to modification to produce safe, highly
selective and efficacious therapeutic anticancer peptides (ACPs).

 

Animal venom becoming an important source for ACPs discovery and development, as many bioactive peptides are
identified from venom, such as melittin and cathelicidin. Recently, mauriporin is
a potential new ACP identified from Scorpion Androctonus mauritanicus, found to exert a dual
pharmacological effects against prostate cancer cells and bacteria with low
hemolytic activities. Although natural ACPs have
a high anticancer activity, but its long amino acid sequence
(30-40 residues) increases the production
costs and could limits its efficacy.

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Therefore, in this study, we will
using in silico method for designing
and predicting several new short ACPs (10 residues) from mauriporin (as a template)
through peptide modification based on amphipathicity, hydrophobicity and
cationic properties using a webserver (AntiCP). Subsequently, in silico pharmacological activities
evaluation of these newly designed mauriporin-derived short ACPs will be further
predicted using other several established webserver platforms to predict its
toxicity (ToxinPrep), membrane penetration (CPP), and hemolytic (HemoPI)
properties. Next, PEP-FOLD will be used as a de novo approach to predict ACPs structures. Lastly, the in vitro validation of these potential designed
mauriporin-derived peptides as potential therapeutic ACPs will be assessed for
its cytotoxicity, hemolytic and apoptosis-inducing effects, in hope to identify
several potent mauriporin-derived ACPs with high anticancer activity and low hemolytic
effect.